comprehensive laboratory animal metabolic system  (Columbus Instruments)

Journal: Frontiers in Physiology

Article Title: Cardiomyocyte crosstalk with endothelium modulates cardiac structure, function, and ischemia-reperfusion injury susceptibility through erythropoietin

doi: 10.3389/fphys.2024.1397049

Figure Lengend Snippet: Greater voluntary wheel running capacity in EPO Δ/Δ compared to EPO fl/fl mice occurred independent from differences in hematocrit and whole-body metabolism. (A) Kidney Epo RNA expression (normalized to β-Actin ) in EPO fl/fl and EPO Δ/Δ mice, (B) serum EPO, (C) hematocrit (%) collected from the saphenous vein of EPO fl/fl and EPO Δ/Δ mice, and (D) average voluntary wheel running distance (km) across 3 days. Comprehensive Laboratory Animal Monitoring System (CLAMS) measured (E, F) VO 2 (mL/kg/min), (G, H) VCO 2 (mL/kg/min), (I, J) RER (VCO 2 /VO 2 ), and (K-L) total energy expenditure (TEE, kcal/hour) in EPO fl/fl and EPO Δ/Δ mice during their sleep and wake phases. An unpaired, two-tailed t-test was used to detect differences. Data are expressed as mean ± SD (for qPCR, serum EPO levels, and hematocrit) or mean ± SEM (for average running distance and whole-body metabolic readings). Data were considered significant when p < 0.05.

Article Snippet: The Comprehensive Laboratory Animal Monitoring System (CLAMS) metabolic caging apparatus (Columbus Instruments Oxymax) is a sealed indirect calorimeter used for the simultaneous measurement of multiple parameters, including oxygen consumption (VO 2 ), carbon dioxide production (VCO 2 ), and calculation of respiratory exchange ratio (RER) across 24-h. EPO fl/fl and EPO Δ/Δ mice were weighed and individually placed into the CLAMS caging for a 24-h acclimatization period followed by a subsequent 24-h data collection period.

Techniques: RNA Expression, Two Tailed Test